Honey is a sweet juice collected by bees from plants. They process it, enrich it with enzymes and store it in honeycombs. The composition of honey generally includes invert sugar, water, dextrin, nitrogenous substances, acids and minerals. Moreover, there are different quantities of amino acids, vitamins - B1, B2, B3, B6, K, C, E and hormones in a honey. Honey has various healing and dietary characteristics and therefore it is used largely in a folk and scientific medicine. In the literature there are different data on the amino acid composition of different honey types varying in different boundaries. All this provokes us to make a qualitative amino acid analysis of several types of honey from some geographic regions of Bulgaria. It was found that the studied samples contain proline, serine, alanine and glutamic acid.